Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 viability. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell viability was determined by MTT assay. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: MTT Assay

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 apoptosis. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide assay. (B) Quantification of cell apoptosis. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques:

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 migration. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell migration was determined by Boyden chamber assay Transwell assay. (B) Quantitative analysis of cell migration. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Migration, Boyden Chamber Assay, Transwell Assay

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 tube formation. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then tube formation was determined by Matrigel tube formation assay. (B) Quantitative analysis of tube formation. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Tube Formation Assay

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on cell adhesion of (A) HUVEC and (B) HMEC-1 to ECM proteins. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then the absorbance of ECM proteins was determined. *P<0.05 vs. control group. PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; ECM, extracellular matrix; Group 1, control.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques:

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC- and HMEC-1-induced ROS production. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then ROS production was determined by 2′,7′-dichlorofluorescin diacetate assay. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; ROS, reactive oxygen species.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques:

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC- and HMEC-1-induced IL-8 and TNF-α expression. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then (A) IL-8 and (B) TNF-α expression was determined by ELISA. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; IL, interleukin; TNF, tumor necrosis factor.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay